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e-DNA Sampling For Sasquatch


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hiflier
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I agree with what you say, MIB, because the general consensus in science seems to say that advances in e-DNA technology isn't so much linear as it is exponential. So many scientists are working on these advancements that I have no doubt a breakthrough that deals with hypotheses such as mine will be sooner than later. In the meantime, is moving forward on some kind of e-DNA connection to a reputable lab still worth the effort? Personally, I think so. I have not heard anything from Genidaqs on my second email so it looks like I will need to call them in the near future.

 

The reason I would like to stay with them is two-fold: They were mentioned by a Bigfoot researcher, and they are located close to Northern California which means sample have a better chance of arriving fresh. I also want to look into them actually being the ones taking the samples since they do offer that. I also think they offer the use of free equipment but I need to nail that aspect down to be sure. They do say that on their website:

 

"Sampling supplies are part of the cost and include filter, end-caps, adapters and tubing. Additional sampling kit equipment (pump, drill) can be borrowed for no-cost, but most clients prefer to own these items. New clients are encouraged to schedule a training session prior to designing their study."

 

This is exactly the kind of set up I was looking for way back when I was suggesting working with academia where they would let citizen scientists use their equipment. So between submitting samples for testing and then the actual cost of testing then anyone wanting to collect samples wouldn't be out-of-pocket when it comes to actually having a set up in the field for doing so.

 

They also say this which is why I need to speak with someone at the company (my bolding):

 

"Please contact us to learn more about creating sampling strategy to increase you PoD (Probability of Detection) by sample replication (how many filters are collected), sampling intervals (spatial and temporal) and sample volume (volume passed through the filter)."

 

Edited by hiflier
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  • 2 weeks later...
hiflier
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I'm going to be calling Genidaq tomorrow and see if I can speak to someone regarding a potential program for us to use in our quest. Also I have a list of questions to ask them and if anyone can think of any they wish me to ask on top of what I already have then let me know. Here's the list:

 

- Are you capable of running such a test?
- Can you access the primers and assays necessary to run species specific testing?
- Would it be better or cheaper to just run a general species protocol?
- What is the cost?
- If the testing is set up, is it better to run one test at a time or wait until a bunch of samples are gathered?
- Is there a cost break for testing more than one sample?
- What equipment or materials are supplied, or available for loan?.
- We already know how to run field samples.
- If an active area is discovered would you prefer water or soil samples?
- What's the turn around time?
- If you get a positive result for a primate other than Human will you tell us?
- By law, do you need to inform someone in authority?
- If positive, can we have enough time to secure legal council before you tell anyone else?
- Do you understand what's at stake should testing show a positive result?
- Would you want to be known as the lab that ran the samples and sent them out for peer review?
- Do you have places that you access for peer reviewing samples of suspected rare species?

- How do you prefer the samples to be shipped to you?

- Chain of custody form to fill out?

- At what point is payment required for services rendered?

- What is your preferred method of payment? 

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NorthWind

Do you think they might sign an NDA? If I submitted positive testing samples, for instance, I don't want the media or anyone else showing up at my door asking me questions. Maybe there is a way to save samples in one location (freezer?) and submit a bunch at a time, rather than one item at a time, sent by each individual. I suggest that if so, they be coded in a manner that would be only identified by a BFF member so as not to reveal details about home addresses or specific location of the find or finds. Just tossing ideas out. 

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SWWASAS
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We have to be careful what we wish for.      The person that somehow gets science interested in existence by DNA or any other method may have a difficult time avoiding publicity.    I have been tracked down  and contacted by  "Finding Bigfoot" TV producers and a web forum host.    I did not initiate or desire either contact.   There are very good reasons for not discovering bigfoot.  

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hiflier
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3 hours ago, SWWASAS said:

The person that somehow gets science interested in existence by DNA or any other method may have a difficult time avoiding publicity.

 

And my response to any media? "No comment".

 

3 hours ago, SWWASAS said:

There are very good reasons for not discovering bigfoot.

 

Yessir, there are. And woe to those who have not thought things through.

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hiflier
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I called, spoke to the secretary, was connected to the person I wished to talk to but got voice mail. Left a message, so looks like perhaps tomorrow if I'm lucky, but more likely first of the week. I did run across a connected website though that listed Genidaq's competitors so there could be options there if things don't work out with this company. Hey, I'm patient. Something will come of this, that, I'm sure of. What is important is that whoever I end up in dialogue with, they need to let me know what I need to do to show that I'm legit. Because I AM legit. So.....we wait.

Edited by hiflier
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  • 2 months later...

This is a bit of a tangent but I thought it was interesting inasmuch as it connects to the idea of finding evidence in the environment:

 

https://www.sciencealert.com/can-the-leaves-of-plants-help-us-find-buried-human-remains

 

The thesis: In the USA, 100 000 people go missing every year. Difficulty in the rapid identification of sites of human decomposition complicates the recovery of bodies, especially in forests. We propose that spectral responses in tree and shrub canopies could act as guides to find cadavers using remote sensing platforms for societal benefit.  Link

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That's wild.

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SWWASAS
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Saw a bigfoot show on Travel channel last night.    Title had something to do with monsters.     Davis,  Medrum,   Smeja and Stacy Brown were interviewed.     Smeja and Brown led a team from some E-DNA lab in California to the site of their mutual sighting in the Willow Creek area.   .    This lab was happy to run E-DNA testing.   They found a number of common animals DNA in the creek running through the area as well as normal and strange human DNA.    The strange stuff was said to be 98% human but had a bunch of strange markers.   But as they mentioned a chimpanzee is 98% too.   I wish I had caught the name of the lab.   Looked like they had no problem will looking for BF E DNA.   They mentioned that the DNA seemed similar to the DNA of the elongated skulls found in Chile they had tested.    They mentioned the ET angle and wondered if BF are the real earth humans and not hybridized aliens like we could be.    Interesting in that I had not seen it and the DNA testing was done in 2018 so fairly recently.    

 

Speaking of ETs,   yesterday morning where I live was in a deep inversion layer.   Freezing fog and a temperature about 26 degrees.   No wind at all and my weather station had not shown any wind for hours.    As I was looking out the kitchen window,   about 12 feet up in a tree at the woodline on the back of my property I could see the branches begin to shake energentically in one part of a tree like something big was in the tree.    The hair stood up on my neck and I went out onto the deck to have a better look.   The shaking had stopped, not a breath of wind, and the tree branches were thin enought I could see through them.     Expecting to see some critter I could not see anything in the tree.    Reminded me of the Predator movie when it was up in a tree.    Spooky.   Glad I was not out in the woods alone when it happened.    Strange stuff happens in the woods.  

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BFF Donor

There was a show on TV a few days ago about the Yeti and an area in or near Bhutan. They were trying to extract eDNA from footprints in the snow and water in ponds.  They found footprints and took samples and took water sample from a pond an stream.

 

In the end, the eDNA revealed the pond had DNA that was 99% human. I found that interesting. If I can find the name of the show, I will post it here.

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hiflier
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I saw that show last Summer. I think it was a Nat Geo documentary called "The Lost Kingdom of the Yeti." If it's the same show you watched, there was an eight person English/French team that included a French e-DNA specialist named Dr. Eva Bellemain. I don't remember the part about finding Human DNA in a pond, but the snow track DNA they collected was bear. She has also worked on Polar Bear DNA in the arctic and took samples one time from a track that showed Polar Bear as well as seal. The conclusion was that the bear must have been following/chasing the seal. So it would seem her specialty is collecting and testing DNA from footprints/paw prints in snow. I did shoot her an email shortly after I watched the program but never heard back.

Edited by hiflier
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On 12/24/2020 at 1:21 PM, SWWASAS said:

Saw a bigfoot show on Travel channel last night.    Title had something to do with monsters.     Davis,  Medrum,   Smeja and Stacy Brown were interviewed.     Smeja and Brown led a team from some E-DNA lab in California to the site of their mutual sighting in the Willow Creek area.   .    This lab was happy to run E-DNA testing.   They found a number of common animals DNA in the creek running through the area as well as normal and strange human DNA.    The strange stuff was said to be 98% human but had a bunch of strange markers.   But as they mentioned a chimpanzee is 98% too.   I wish I had caught the name of the lab.   Looked like they had no problem will looking for BF E DNA.   They mentioned that the DNA seemed similar to the DNA of the elongated skulls found in Chile they had tested.    They mentioned the ET angle and wondered if BF are the real earth humans and not hybridized aliens like we could be.    Interesting in that I had not seen it and the DNA testing was done in 2018 so fairly recently.    

 

Speaking of ETs,   yesterday morning where I live was in a deep inversion layer.   Freezing fog and a temperature about 26 degrees.   No wind at all and my weather station had not shown any wind for hours.    As I was looking out the kitchen window,   about 12 feet up in a tree at the woodline on the back of my property I could see the branches begin to shake energentically in one part of a tree like something big was in the tree.    The hair stood up on my neck and I went out onto the deck to have a better look.   The shaking had stopped, not a breath of wind, and the tree branches were thin enought I could see through them.     Expecting to see some critter I could not see anything in the tree.    Reminded me of the Predator movie when it was up in a tree.    Spooky.   Glad I was not out in the woods alone when it happened.    Strange stuff happens in the woods.  

 

 

Smeja is in norcal and stacy brown is in Florida so they would not be part of the same site unless someone funded a Bigfoot team-up.  Would National Geographic do such a thing? :popcorn: Also, I would draw the line at DNA of elongated skulls. That is different than BF and no reason to go down that rabbit trail or deal with alien theories with eDNA. I am curious what DNA lab states a potential link with aliens?  I will have to check out the documentary.

Edited by Arvedis
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@hiflier  I think this was a different one. That same French woman was in this documentary too but I don't remember any bear results. The two surprising conclusions---One was DNA identified as Argali which is the largest member of the sheep family. 400lbs. It was not thought to be in that region. The other, and biggest IMO, was DNA from the pond was 99% human.

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hiflier
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4 hours ago, wiiawiwb said:

@hiflier  I think this was a different one. That same French woman was in this documentary too but I don't remember any bear results. The two surprising conclusions---One was DNA identified as Argali which is the largest member of the sheep family. 400lbs. It was not thought to be in that region. The other, and biggest IMO, was DNA from the pond was 99% human.

 

Cool, w, let me know if you come across a link for that one. In any case, either my email was simply deleted upon arrival or it was read and ignored. I think it's important to reach out to these people whenever possible because one never knows, especially since Dr. Sykes is no longer with us.

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I've been watching my cable listings to try and find it. The show definitely had "Yeti" in the name.

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