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  1. Today
  2. I am so sorry for your loss I hope you’re doing well I know what it is to love your companion I have a little one as well and she’s also 14 I wouldn’t know what I do without her take care and be safe
  3. gigantor

    e-DNA Sampling For Sasquatch

    It depends on how much dna of the subject there is in the water. If you get water from a running River, there's probably very little of it and you would need to filter a lot of water, hundreds of gallons or more. If you get water from a puddle where Bigfoot just took a leak, then a jar of water would be enough. Something like that.
  4. PNWexplorer

    Who To Learn From

    I wonder if it would helpful as well to zero in on researching Bigfoot behavior in your specific region, as it seems they vary wildly in behavior/food sources/habits/physical characteristics in different parts of the country.
  5. norseman

    e-DNA Sampling For Sasquatch

    I would be curious to know when they discover a new species or even detect extant ones? How many liters of water does it take on average? Are we talking 10/100/1000/10x100?
  6. Yes, it is in .45 Colt and loaded with 300 grain hard cast bullets I cast and loaded myself. I've since retired it in favor of an AR15 I built in .450 Bushmaster.
  7. gigantor

    e-DNA Sampling For Sasquatch

    Great job Hiflier! If you want detection you need: Nanogram = billionth of a gram Microliter = Millionth of a liter 1000000 x 0.000000001 = 0.001 grams per millionth of a liter. That's a lot concentration me thinks. A millionth of a liter is pretty small I suppose. We would have to filter a lot of water.... We could also do this in soil if we find a nest.
  8. PNW - Sorry to hear about you pooch. Nothing worse than losing a loved one. It's a wonderful picture to cherish. Your lever gun should be able to dissuade packs of wolves. Is it 45 Colt?
  9. norseman

    e-DNA Sampling For Sasquatch

    Exciting!!! What I’m reading is that if they know what they are looking for (unknown primate) they can adjust their studies to accommodate the search? So.....there IS a way forward concerning this research! By high volume? How much water are they talking about I wonder?
  10. I'm also sorry to read of your loss. There is no love like the love of a dog.
  11. Yesterday
  12. hiflier

    e-DNA Sampling For Sasquatch

    You, sir, would bring a tear to my eye I probably should take this opportunity to present a couple of updates. These are responses that I have received regarding my NOTCH2NL concept: 1) "It would be a great study, but I am afraid you will need a good fresh sample of blood or tissue or bone to extract enough nDNA to sequence these genes. I read about the genes and had no other thoughts. Best of Luck. If you get an adequate sample you should try a whole genome sequencing to answer many more questions." Followed up response: "Your genes are nuclear genes, but there is a 1000+X advantage in mtDNA, so much better chance to capture in an e DNA experiment. It doesn't hurt to look, however. I do think that a well designed eDNA project involving collection of water samples could reveal an unknown primate." And this is a response from a different source. A lab in fact: ".....there are two technical issue that impacts your suggested approach. 1) Species ID approaches typically use mitochondrial DNA, as there can be dozens to hundreds of mitochondria per cell. Importantly, this means that mtDNA is at least one (but probably more) order of magnitude more common in the environmental than nuclear DNA. Given rare species, by definition, are at low density, their DNA in any given environmental sample is present in low concentration. For quantitative PCR, the limit of detection is around 1x10^-5 or 1x10^-6 nanograms per microliter. The concentration of mtDNA for rare species is typically at or below limit of detection, so we have to sample in ways to get the DNA concentration above the detection threshold. High volume filtered, technical replicates etc. We are investigating ways to reliably use nuclear DNA from environmental samples, but this isn't something we recommend at this time. 2) Environmental samples are "pools" of all the DNA present. Many different organisms and/or multiple individuals of the same organism. So you either have to target the species of interest using qPCR or you have to conduct a metabarcoding analysis, which separately analyzes each DNA strand present. As there is no qPCR assay specific to Bigfoot, metabarcoding is the only choice for water samples. [Soil is a different story] Currently, given the sequencing technology, I think you'd have to conduct meta (data), but use the NOTCH2NL marker for amplification to pull the locus of interest from the "pool" of DNA. We can process disc filters, although we recommend sterivex filters for rare DNA at low concentration." So, as you all can see, I have been trying to do my part as far as laying the groundwork, not only to find support for the idea, but to also find a facility that would be open to testing samples. Something that I'm continuing in doing because one lab may not be sufficient if any samples succeed in getting a positive result. So far there seems to be some good support for the NOTCH2NL approach. It has taken months to nail down positive responses like these. Nearly all inquiries have otherwise gone ignored.
  13. ^ Who knew one could make a decent living that way....
  14. So sorry to hear about your companion. There's nothing like 'em.
  15. BobbyO

    e-DNA Sampling For Sasquatch

    Ask and you shall receive !
  16. Eric Brown is the king of short, violent, gory Bigfoot novels.
  17. BlackRockBigfoot

    For believers; do family/friends believe/support you?

    I am sorry for your loss as well. He looks like a big happy boy.
  18. It is not as good as WWZ. The core of it is a bigfoot horror movie script that Mr. Brooks sold some years ago. The idea kept tickling him so he get the rights for the script back when nothing happened and wrote this book. I enjoyed it. It may be the best piece of bigfoot fiction I've read, but it is not the bench-mark that WWZ was. People get eaten. It is gory. It is definitely a bigfoot horror story. I have read one of Eric Brown's books. It was OK, but a bit too pulpy for my personal taste. The gory level was probably higher and the development of characters and situation less involved.
  19. Sorry for your loss. Mot people don't understand a bond with a Dog.
  20. I had an old pop-up camper I restored the last few years and took it out in the woods solo with just me and my best friend, a 100lb Great Dane/Lab mix. It was perfect for us and having him around really eased my mind. Sadly, he had to be put down last week after developing severe arthritis and a brain tumor at nearly 14 years of age. To be honest, the idea of being totally alone in the wilderness at night with no furry or non-furry companion is terrifying to me. I'm kind of a big sissy when it comes to the dark and the woods, lol. Last time I was out, some wolves came and checked out me and my dog. We both stayed close to the fire...
  21. TC Lowside

    Who To Learn From

    Great thread! Very helpful. I now have a reading list to pursue and a list of what not to waste time on.
  22. hiflier

    Historical Research

    Bigfoot- Man or Beast: It was interesting to see credits at the end with the different gov. agencies and organizations that supported the expedition. Wouldn't happen in this day and age? Really doubt it would.
  23. Todd Prescott

    Historical Research

    Here is a continuation of Sasquatch: The Anthropology of the Unknown (from 1978).
  24. Todd Prescott

    First Sasquatch Conference audio and video

    Here is the continuation of Part 1 as posted above.
  25. Redbone

    Historical Research

    "Bigfoot - Man or Beast" (1971)
  26. Wow, what a gorgeous view. I would encourage you to consider an overnight stay. That's when the action will most likely come alive. Even if you go in late in the day (or even early evening) and leave the next day at the crack of dawn. Maybe only in the woods 12 hours, or less, but your focusing your efforts on the most productive time. I've often used the SSR, here at BFF, and have found that in my area the most productive time for sightings, wood knocks, and other evidence is between 9pm and 11pm. Oddly enough, my best events, or pieces of evidence, happened within that same time frame. Your area my be slightly different.
  27. When I found a sasquatch trackway I was with several other guys, including my brother. I've lost contact with the other guys, and frankly only clearly remember four of the other guys who were there, two of which I could't find if I had to (I can't even remember one if their names). My brother doesn't remember the find at all, but really, that isn't very remarkable at all. He has an incredibly poor memory. When I and others talk about childhood friends, he's always at a near complete loss. He's constantly contacting me asking if I remember somebody who he has re-met and who remembers him. But I agree that some folks probably have some sort of memory regression involving a sasquatch experience due to discomfort or fear.
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