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Showing content with the highest reputation on 04/18/2023 in all areas

  1. I'm happy to announce that we may be seeing some bold new PGF research, because Doug Hajicek (of MonsterQuest fame) is launching a new funding campaign for "Legend Meets Science 2" as seen here: https://www.kickstarter.com/projects/hajicek/legend-meets-science-ii-film-sequel-a-bigfoot-documentary?ref=3icq5m&token=6ab15f4a Doug did an incredible job on LMS 1, providing researchers with some of the best frame scans and video renders of the PGF for many years, and he and I have been in talks for some time now about taking PGF research to the next level in this new LMS 2 project. So please support it, or share the info with friends, and if it is successful, we can look forward to some bold and exciting new research. Mods: I hope this notice is appropriately placed. If not, move at your discretion. Thank you.
    4 points
  2. I do not go out without snake boots.
    1 point
  3. Continuing.... Metabarcoding inthe lab picks up the genus of everything that visits or is currently living is an environment. Everything. Dr. Mayor's did. But she also got genus Pan troglodyte (Chimp) DNA, and, more than likely Human DNA as well. What did Dr. Melderum get. Everything also. With one exception: Human DNA that was apparently too degraded to show maybe Pan troglodyte or some other primate? Both of which are different of a different genus. But nope, same kind of environment with regards to rainfall. And again samples from soil. In Dr. Meldrums case the soils was even more protected from UV rays because the soil was under the nest structures but perfectly capable of showing an organisms genus. How do I know? Because the genus of all the animals that had visited or lived close by turned up in the results. But only ONE was apparently too degraded to show anything other than Human. But t did show genus Homo (Human), right? And the genus of everything else showed up as well. DNA sequencing at that time was very precise and since the announcements by Dr. Meldrum and Dr. Disotell came at about the same time in the Fall of 2018, only about for years ago, those announcements came soon after the lab sequencing and results were known. Science had already been using environmental DNA sequencing for a couple of decades before those announcements were made to identify an animal's genus in the wild. It was not a new science by any means by the time those soil samples were collected and tested. Dr. Mayor's in 2020 and Drs. Meldrum and Disotell's in 2017 or 2018. So, all being equal where environment, rainfall, professional collection and sequencing protocols, are in line, what gives with one getting a non-Human genus in it sample and the other not? The Human genome is the gold standard, the reference that is used in which all other DNA sequences are compared to. It is the base line and is constantly being refined as more and more Human genomes get factored in to improve it as a reference genome. The smallest DNA molecule is the mitochondrial but it can have hundreds of copies of itself inside a single cell. And though small, it STILL contains over 16,500 (16,569 to be exact) amino acid pairs. How many, out of those 16,569 amino acid pairs, are needed to determine genus match? Less than 50. Sometimes even only 20. Ideally an unbroken sequence of 150 pairs is ideal and considered a good quality sample. For determining genus that's all it would take. Good enough for Dr. Mayor Kentucky samples submitted to the lab at the University of California. But somehow Dr. Meldrum's from Mason County was too degraded for Dr. Disotell's state-of-the-art lab at the time at NYU? He was, and still is after all, an expert in evolutionary primate genetics, though he has since relocated to UMass Anherst in the fall of 2019. Now I'm no DNA expert but I AM a logician and this one loose end has never made any sense to me whatsoever. Why doesn't it make sense? Because no one knows how long Dr. Mayor's large wood structure was there in eastern Kentucky. And no one knows how long that Chimp DNA was there either. But one thing is certain, the nest structures in Mason county were fresh constructs. Vertical stems were pushed into the ground. All the material for the nests were harvested close by and woven into those nests. So regardless of the soil under the centers of them, there should have been DNA left all over the place there: Under and around these structures on the ground, on the ground in the huckleberry patches and on the stems. DNA should have been just about anywhere that could have been sampled. So what happened? No one thought of that? DNA is pretty tough stuff. It's not going to go away or completely degrade after only a few weeks or months. Everything I've said so far in these first three posts is all scientific FACT, along other information as it was reported by the parties involved in these discoveries. Fact, Sasquatch DNA won't be Homo Sapien DNA that is closer than Neanderthal or Denisovan or any other recent Human cousin. It's DNA MAY be Homo but it would be so far removed genetically that there would be no chance of mistaking it. In other words, it would have so many mutations as to be unrecognizable as Modern Human no matter how degraded. No one can tell me that out of 16,569 amino acids that none of it would show non-Human mutations. It borders on the ridiculous to think otherwise. The ONLY conclusion that one can arrive at that makes any sense is that Sasquatches did NOT build those structures. But all the manually broken stems of the huckleberry bushes suggests that it wasn't Humans. But if it wasn't Humans then someone is covering up the fact that there must have been Non-Human primate DNA at that Mason county site. The point is, there is no way, when all other animal genera were there, that only Human DNA was found to be degraded, at a site with every chance possible to have DNA fresh enough to show a non-Human primate was present. There. Done. Does anyone agree or disagree with this assessment?
    1 point
  4. Okay, eastern Kentucky. Why there? Maybe to meet up with Adrian Erickson? Maybe not? The point here is that according to some studies over the years by people working on the Sasquatch issue have postulated that the creature seems to prefer a climate that sees 30-40 inches, or more, of annual rainfall. Eastern Kentucky has an annual rainfall of 38 inches. Southeaster Kentucky can average as much as 58 inches. That's about as much as the Ouachita National Forest which has an average annual rainfall of a little over 50". Mason County in Washington state hovers between 40-50 inches a year. These are important facts to know for this thread because it goes to what one might expect with regards to the quality of DNA collected, even though time and other influences can be a factor affecting that quality. No brainer there, right? But here's where it gets interesting. Mason county, the Ouachita NF, and eastern Kentucky average out about the same on annual rainfall. Dr. Mayor got Chimp DNA (genus Pan troglodyte) from the soil under a wood structure. No one knows how long that structure was there or when a "Chimp or Chimps" visited the site. But regardless the DNA in the soil was good enough quality to show genus. But what about the nest structures in Mason County, Washington? Soil samples were taken there as well and apparently, according to Derek Randles and others, there was still greenery (leaves) on some of the material used to build the structures. So. Fresh structures only a few months old. Dr. Jeffrey Meldrum was called in and he took soil samples from under the centers of the nests. The same protocols ere followed in the lab by Dr. Todd Disotell. And I know that to be true because, like eastern Kentucky, the sequencing process picked up on everything at the nest site that on would expect. In other words, ALL of the organisms that were expected and that had their genomes in the GenBank. So bottom line here is that both Mason County and eastern Kentucky receive approximately the same amount of rainfall, both had some kind of manufactured structure or structures, both had professional PhD's take soil samples, and both ran those samples using a lab protocol utilizing next generation, high throughout, metabarcode sequencing. Both got multiple organisms returned in the results and yet.... Break time.....
    1 point
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